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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and thus may possibly add to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important job in endorsing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two added splice variants have been noted but in depth nucleotide sequence details isn't yet readily available.
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Blocking peptides are peptides that bind exclusively towards the focus on antibody and block antibody binding. These peptide generally consists of the epitope regarded from the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to ensure the very best success are...
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This guidebook outlines even further possible optimization of cellular and immunoassay parameters to ensure the absolute best final results are...
Antibodies bound to the blocking peptide not bind to your epitope around the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Target Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show unique expression and degradation styles which lead towards the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features for a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is degraded as cells progress by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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